Accession Number:

AD0684245

Title:

OPTIMIZATION OF A SYNTHETIC NUTRIENT MEDIUM FOR CULTURING TYPHOID BACILLI. REPORT 1: SELECTION OF THE MOST ESSENTIAL FACTORS OF A NUTRIENT MEDIUM BY THE METHOD OF ACCIDENTAL BALANCE,

Descriptive Note:

Corporate Author:

ARMY FOREIGN SCIENCE AND TECHNOLOGY CENTER WASHINGTON D C

Personal Author(s):

Report Date:

1969-02-18

Pagination or Media Count:

12.0

Abstract:

The Satterthwaite random balance method was employed to select an optimum nutrient medium for culturing typhoid bacteria. The addition of five supplementary nutrients dl-aspargic and dl-glutamic acids, dl-lysin, dl-methionine and l-cistine to the Troitskiy-Gekker synthetic medium resulted in a six-fold increase in microbial mass. On the basis of the Satterthwaite method it was determined that the five factors most influencing the intensity of propagation were l-tryptophane, dl-asparagic acid, glucose, bisubstituted ammonium citrate and nicotinic acid. Dl-lysin was omitted from the medium because of its competitive interaction with nicotinic acid. Author

Subject Categories:

  • Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE