Accession Number:

AD0684175

Title:

COATED CHARCOAL RADIOIMMUNOASSAY FOR PLASMA INSULIN,

Descriptive Note:

Corporate Author:

SCHOOL OF AEROSPACE MEDICINE BROOKS AFB TEX

Report Date:

1968-11-01

Pagination or Media Count:

13.0

Abstract:

A radioimmunoassay using coated charcoal was developed as a simple, precise, and reproducible technic for measuring plasma insulin. Materials used included insulin-125I, guinea pig anti-insulin serum, and coated charcoal. Varying reagent volumes were tested to achieve an antibody concentration which would bind 60 to 80 of the labeled antigen. The radioimmunoassay consisted of three phases 1 addition of reagents and a 60-hour incubation period, 2 separation of the antibody-insulin complex from the unbound insulin, and 3 determination of activity and calculation of the insulin concentration. The fasting value found with 10 control subjects ranged from 12 to 72 micro-U.ml. This procedure is adaptable for use as a routine diagnostic test by most radioisotope laboratories. Author

Subject Categories:

  • Medicine and Medical Research
  • Radiobiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE