A SIMPLIFIED DETERMINATION OF URINARY TESTOSTERONE UTILIZING COLUMN AND GAS-LIQUID CHROMATOGRAPHY.
Rept. for Jul 66-Jul 68,
SCHOOL OF AEROSPACE MEDICINE BROOKS AFB TEX
Pagination or Media Count:
A simplified and reliable assay for urinary testosterone has been established which requires only one purification by silica gel column chromatography prior to quantification by gas chromatography. The procedure avoids most of the enzyme-inhibiting debris in the urine by preliminary precipitation of the steroidal material with ammonium sulfate. Enzyme hydrolysis reaches near-maximum completion in 18 to 24 hours at 37C. with only 10 the normal dosage of enzyme. Author