A STUDY OF THE BIOLOGICAL PROPERTIES OF TRYPANOSOMA RHODESIENSE AND TRYPANOSOMA GAMBIENSE
Annual progress rept. no. 1, 1 Nov 1966-31 Oct 1968
TULANE UNIV NEW ORLEANS LA DEPT OF BIOLOGY
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Three enzymes, hexokinase, aldolase and phosphohexoisomerase, were partially purified by a combination of NH42SO4 fractionation, and DEAE-Sephadex column chromatography. These enzymes have been biologically and physically characterized. Since these enzymes are critical trypanosome enzymes, neutralizing rabbit antisera prepared against them have been tested for their ability to passively protect mice. No protection was observed. In addition by similar techniques a protective antigen was partially purified from crude extracts of Trypanosoma gambiense. One of the most significant advances made by these studies was the development of a working hypothesis to account for the death and pathology of T. gambiense infected animals. In synopsis, this hypothesis involved the presence of a trypanosome toxin, delayed-type hypersensitivity and auto-immunity.
- Anatomy and Physiology