A STUDY OF TETANUS TOXINS AND TOXOIDS PREPARED IN CASEIN MEDIA
ARMY BIOLOGICAL LABS FREDERICK MD
Pagination or Media Count:
Tetanus toxins prepared in casein media have a toxicity of 2 or 3 times greater strength than toxins prepared in Ramon and Gluzman media. Tetanus toxoids prepared from casein media have an antigen content 2 or 3 times greater than toxoids prepared from meat media. The inactivation of tetanus toxins prepared in casein media can be achieved in a shorter time than the inactivation of toxins prepared in meat media. The fixation capacity of tetanus toxins prepared in casein media ran parallel to the fixation capacity of the toxoids expressed in F.U. fixation units. The immunizing properties of crude tetanus toxoids prepared in casein media satisfied the standards laid down in the instructions for the preparation of tetanus toxoids in meat media. Re- investigation carried out 1 year after preparation showed that the antigen content and antigenicity of toxoids prepared in casein media was fully retained. Crude tetanus toxoids prepared in casein media are well adsorbed by aluminum hydroxide. The immunizing properties of tetanus toxoids adsorbed on aluminum hydroxide are higher than the immunizing properties of crude toxoids.