VIRULENCE AND ANTIGENICITY OF A STRAIN OF BACILLUS ANTHRACIS CULTIVATED AT 45 DEGREES. II
ARMY BIOLOGICAL LABS FREDERICK MD
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On cultivation of B. anthracis at 45C the virulence was reduced to the level of virulence of vaccine strains. A broth culture of B. anthracis cultivated at 45C lay as regards virulence between the first and second of Tsenkovskys vaccines and possessed high immunizing properties after a single injection rabbits acquired a high degree of immunity. On prolonged passage of a 7 day culture on to artificial nutrient media, and also repeated passage in mice, the newly acquired properties were not changed. Therefore this method of attenuation can be used for the purpose of preparing an anthrax vaccine.
- Animal Husbandry and Veterinary Medicine