ERYTHROCYTE METABOLISM AND SHAPE TRANSFORMATION IN PRESERVED ERYTHROCYTES.
Final rept. 30 Apr-30 Aug 68,
BROWN UNIV PROVIDENCE R I
Pagination or Media Count:
Erythrocytes of whole blood incubated at 37C underwent large increases in average density and cellular hemoglobin indicative of volume decrease soon after depletion of ATP. A synchronization among the variables, ATP depletion, cation leak, and volume contraction was found which could be accelerated or delayed depending on preservation conditions. In contrast to erythrocytes of whole blood, erythrocytes resuspended in 0.15M NaCl did not contract when ATP was depleted at 37C. The previously observed large K-Na exchange between the cells and the medium initiated by the total loss of ATP was also not observed. However, Ca initially present, or subsequently added produced contraction of cell volume when ATP became reduced 90. It was concluded that Ca normally present in plasma even from ACD or resin collected blood, and very low in erythrocytes must be prevented from entering the cells, or contraction will occur. Appropriate means to prevent Ca entry may be required for the maintainance of cell shape during blood preservation. Author