GROWTH OF PLANT CELL CULTURES. 2. NUTRIENT REQUIREMENTS AND THEIR ROLE IN THE GROWTH OF SUSPENSION CULTURES
ARMY NATICK LABS MA FOOD LAB
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A detailed study has been made of some nutrient requirements of several plant cell suspension cultures. These cultures require the usual inorganic salts including inorganic nitrate nitrogen. Hydrolyzed protein or amino acids will serve as the sole source of nitrogen for growth, but organic nitrogen is not required. Urea or ammonia nitrogen in the absence of nitrate will not support growth. Sucrose is an excellent carbon source. Glucose and starch will also support growth of some cultures. Sucrose sterilized by gamma irradiation supports normal growth of these cultures. Growth is retarded on glucose sterilized by gamma irradiation at ambient temperature, but growth is normal on glucose irradiated at -80C. A few growth factors at low concentration are also required. An auxin 2,4 dichlorphenoxyacetic acid or naphthaleneacetic acid and thiamine are required by all cultures. Kinetin 6 furfurylaminopurine is also required by some cultures. Maximum productivity on this simple medium is about 1.5 grams dry weight per liter of culture per day, equal to, but not significantly greater than productivities obtained on much more complex media.