DISTRIBUTION OF A TRACER PREPARATION WITH BOTULINAL TOXIN IN THE ORGANISM OF WHITE MICE
ARMY BIOLOGICAL LABS FREDERICK MD
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The labelled preparation of the B type botulismotoxin was obtained from the cultural fluid after growing Cl. botulinum on a medium containing S35-methionine. The preparation was purified by a two-fold precipitation with ammonium sulfate and subsequent dialysis in the running fresh and distilled water. The obtained preparation of dry tagged botulisomotoxin was radioactive in the range of 1 mcmg, while its toxicity equalled 5,000 Dlmmg. The distribution of the labelled preparation was studied on white mice every 20, 60 and 150 minutes after an intravenous injection of 1,000 Dlm. The investigation covered blood, brain, muscles, liver, kidneys, lungs, heart and intestines. The toxin preparation was found to rapidly find its way from the blood flow into body tissues. The highest radioactivity rate at all periods of the intoxication was recorded in the lungs, and the lowest - in the blood. During 150 minutes after intravenous administration of the tracer preparation with botulinal toxin a redistribution of radioactivity with a periodic decrease of the tracing concentration in some organs and with an increase in other organs was observed.