METHOD OF PROCESSING BACTERIOLOGICAL PREPARATIONS WITH fluorescent antibodies
ARMY BIOLOGICAL LABS FREDERICK MD
Pagination or Media Count:
The work deals with preparation, fixation and staining of bacteriological preparations with fluorescent antibodies direct and indirect methods. The authors investigations have demonstrated that the temperature regimen and the duration of smear staining by means of fluorescent antibodies are important factors, conditioning the intensity and specificity of microbial cells fluorescence. The composition of the washing liquids and the methods of fixation of the preparations had no essential effect on the capacity of bacterial cells to be stained with specific fluorescent antibodies.
- Medicine and Medical Research