A RAPID METHOD OF OBTAINING ANTIBODIES LABELLED WITH FLUORESCENT STAINS
ARMY BIOLOGICAL LABS FREDERICK MD
Pagination or Media Count:
This report describes a new method for the isolation of immune euglobulins antibodies from agglutinating anti-plague and anti-cholera serums and a new method of purifying the labelled antibodies from the fluorochrome excess. With the new method of isolating and purifying antibodies, the time required to prepare luminescent conjugates is shortened from 3-4 weeks to 3-4 days. The suggested method of preparing fluorochrome-labelled antibodies is realizable in any laboratory, since it requires no special equipment refrigeration centrifuges and rooms. The advantages of a new luminescent stain -- fluorescein isothiocyanate, over fluorescein isocyanate are shown. The immune euglobulins isolated by the new method were classifiable among the gamma- globulins in their electrophoretic composition.