Accession Number:

AD0678896

Title:

A RAPID METHOD OF OBTAINING ANTIBODIES LABELLED WITH FLUORESCENT STAINS

Descriptive Note:

Corporate Author:

ARMY BIOLOGICAL LABS FREDERICK MD

Report Date:

1968-07-01

Pagination or Media Count:

9.0

Abstract:

This report describes a new method for the isolation of immune euglobulins antibodies from agglutinating anti-plague and anti-cholera serums and a new method of purifying the labelled antibodies from the fluorochrome excess. With the new method of isolating and purifying antibodies, the time required to prepare luminescent conjugates is shortened from 3-4 weeks to 3-4 days. The suggested method of preparing fluorochrome-labelled antibodies is realizable in any laboratory, since it requires no special equipment refrigeration centrifuges and rooms. The advantages of a new luminescent stain -- fluorescein isothiocyanate, over fluorescein isocyanate are shown. The immune euglobulins isolated by the new method were classifiable among the gamma- globulins in their electrophoretic composition.

Subject Categories:

  • Biochemistry
  • Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE