DETERMINATION OF MICROQUANTITIES OF CYSTEINE AND GLUTAHIONE BY THE IODIDE-PERSULFATE REACTION
ARMY BIOLOGICAL LABS FREDERICK MD
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A micromethod of determining thiols of low molecular weight is proposed. The method is based on measurement of the reaction time of the oxidation of the sulfhydryl groups by the iodine liberated upon the reaction of potassium iodide with potassium persulfate. Minimum amounts of the substance which can be determined are 0.8 micrograms for cysteine and one microgram for glutathione.
- Physical Chemistry