EXPERIMENTAL EVALUATION OF NEW LABORATORY METHODS FOR THE STUDY OF VIRUS OF POLIOMYELITIS WITH THE AID OF TISSUE CULTURES
ARMY BIOLOGICAL LABS FREDERICK MD
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The multiplication of poliomyelitis types I, II and III viruses in cultures with surviving and growing tissues brain, skin and muscle of human embryo has been established. The virus of poliomyelitis, during multiplication of tissue cultures, caused a characteristic necrosis of the cells. The cytopathogenic action of the virus of poliomyelitis was easily established during inspection under a microscope of the cultures with young growing cells. After the neutralization of the virus with a typospecific immune serum this effect disappeared. The possibility of typing the virus with a reaction of neutralization in the tissue culture was confirmed and determination of the type of strains of virus, isolated from excrement of ailing children in cultures in revolving tubes, was also accomplished. In tests of cultivation of the poliomyelitis virus type II and IV in cultures of embryonal tissues of cotton rats and white mice and also in the acetic carcinoma of white mice it was impossible to establish multiplication of the virus. The obtained results fully confirm the great advantages of the methods of cultivating virus of poliomyelitis on growing tissues in revolving tubes, allowing for the interchange in numerous studies of the tests of animals.