Accession Number:

AD0673247

Title:

GROWTH OF HIGH-TITRE SEMLIKI FOREST VIRUS IN CONCENTRATED SUSPENSIONS OF CHICK EMBRYO CELLS,

Descriptive Note:

Corporate Author:

MICROBIOLOGICAL RESEARCH ESTABLISHMENT SALISBURY (ENGLAND)

Personal Author(s):

Report Date:

1967-10-02

Pagination or Media Count:

8.0

Abstract:

Freshly prepared chick embryo cells have a lower glycolysis rate than cultured cells and can be used at higher concentrations in suspension to produce virus. Conditions for producing maximal yields about 1000 p.f.u.cell of Semliki Forest virus from suspensions containing 10 to the 7th power to 10 to the 8th power cellsml. were 1 suitable preparation of the cells, 2 incubation at 31C, 3 maintenance of pH at 7 to 7.4, 4 adequate glucose, 5 adequate oxygen, 6 gentle agitation. The cultures required no nutrient other than glucose and the cells were suspended in Earles saline. For cell concentrations up to 3 x 10 to the 7th powerml. cultures were stirred in a simple sealed vessel and buffered with NaHCO3CO2. Cultures containing 10 to the 8th power cellsml. were stirred in a vessel equipped with automatic pH control and produced 10 to the 11th power p.f.u.ml. of virus. Author

Subject Categories:

  • Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE