GENE TRANSFER IN STRAINS OF PASTEURELLA PSEUDOTUBERCULOSIS
FORT DETRICK FREDERICK MD
Pagination or Media Count:
Pasteurella pseudotuberculosis strain 32IV accepted the Flac episome from Escherichia coli strain 23.IOS and behaved as a gene donor in crosses with several different auxotrophs of P. pseudotuberculosis. Some selected donor markers were transferred at frequencies of .0001 to .00001 per donor cell while others appeared not to be transferred. Up to 40 of recombinants were Lac. Selected recombinants showed differing unselected marker frequencies with differing selected markers those obtained by using double marker selection showed increased unselected marker frequencies. Some alternative explanations for the origin of recombinants syntrophic growth, mixed clones, multiple recipient reversions were not supported by experiment.