PHAGE REPRODUCTION IN PENICILLIN TREATED ENTERIC BACILLI CELLS
ARMY BIOLOGICAL LABS FREDERICK MD
Pagination or Media Count:
The protoplast-like forms produced from enteric bacilli by the effect of penicillin are capable of adsorbing phage, and are thus distinguished from the protoplasts produced through the use of lysozyme. The protoplast-like forms are capable of securing the reproduction of phage, although the phage production is ten times less than that from normal cells. The penicillin does not affect the amount of phage adsorption, but suppresses the intracellular phase of the phages development in the spherical bodies. Therefore, in a study of the given process it is necessary to remove the penicillin from the medium in which they are situated. Inasmuch as the protoplast-like formations are capable of securing phage development and at the same time, evidently, possess an increased permeability for the macromolecular substrates, their use seems expedient for the study of the reproduction mechanism of bacterial viruses.