Accession Number:

AD0673019

Title:

THE GROWTH OF JAPANESE AND WEST NILE ENCEPHALITIS VIRUSES IN TISSUE CULTURES,

Descriptive Note:

Corporate Author:

ARMY BIOLOGICAL LABS FREDERICK MD

Personal Author(s):

Report Date:

1968-07-01

Pagination or Media Count:

8.0

Abstract:

The cytopathogenic effect, which was registered from the first passage, was regularly detected in the cultivation of three strains of the JE virus Ya-47, R-1, K-2 and one strain of the West Nile encephalitis virus in cultures of monkey-heart cells. Practically identical results were noted in a parallel titration of the virus in tissue cultures and in mice. The specificity of the viruss cytopathogenic effect was proved by means of extinguishing it with a homologous immune serum. In the HeLa cells a specific cytopathogenic effect was registered after six cultural passages of the strain R-1. The cytopathogenic effect was retained in subinoculation. The West Nile encephalitis virus induced a cytopathogenic effect in the HeLa cells from the first passage. The Japanese encephalitis virus strains Ya-47, R-1, K-2, K-4, and Nakayama and the West Nile encephalitis virus propagated in the cultures of skin-muscle tissue of the human and chick embryos, but they did not induce a cytopathogenic effect. The mean titer and the viruses in an intracerebral inoculation of mice lg LD50 was 5.6 and 5.0 respectively. Author

Subject Categories:

  • Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE