Accession Number:

AD0672966

Title:

THE USE OF THE METHOD OF REDUCTION OF METHYLENE BLUE FOR THE RAPID DETERMINATION OF THE LIVING BACTERIA COUNT WHEN GROWING THE PLAGUE MICROBE BY THE DEPTH METHOD IN THE REACTION TANK

Descriptive Note:

Corporate Author:

ARMY BIOLOGICAL LABS FREDERICK MD

Personal Author(s):

• Liskina, I. V.

Report Date:

1965-10-15

Pagination or Media Count:

10.0

Abstract:

There is a direct correspondence between the living microbe count determined by the cultural method, and the discoloration rate of methylene blue at individual phases of growth of the plague microbe in the reaction tank. The method of reduction of methylene blue can be used for the rapid determination of living microbes in the process of growth of the plague microbe in a reaction tank under the aeration conditions. The additional nutrition of the plague microbe with glucose in the process of growth of the plague microbe, which results in a sharp decrease of pH of the culture medium, disrupts the established direct correspondence between the discoloration rate of methylene blue and the living microbe count, determined by the cultural method.

Descriptors:

• *YERSINIA PESTIS
• USSR
• COUNTING METHODS
• ADDITIVES
• TRANSLATIONS
• METHYLENE BLUE
• REDUCTION(CHEMISTRY)
• PH FACTOR
• GLUCOSE
• GROWTH(PHYSIOLOGY)
• CULTURE MEDIA

Subject Categories:

• Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE