Accession Number:

AD0672819

Title:

FLUOROMETRIC DETECTION OF BIOLOGIC CHANGES IN IRRADIATED LABORATORY ANIMALS.

Descriptive Note:

Final rept. Oct 66-Nov 67,

Corporate Author:

NORTHROP CORPORATE LABS HAWTHORNE CALIF LIFE SCIENCES LABS

Personal Author(s):

• Demetriou,James A.
• Beattie,John M.

Report Date:

1968-05-01

Pagination or Media Count:

30.0

Abstract:

The excretion of fluorescent products by rodents and primates after exposure to whole-body sublethal 60Co gamma irradiation was investigated. Gel filtration chromatography and thin-layer chromatography were used to fractionate and quantitate radiation-responsive fluorescent urinary products. A major advancement in quantitating fluorescent urine products was achieved by the fluorescence-scanning of thin-layer chromatograms. Certain radiation dose-response relationships were measurable in two major fluorescent fractions from gel filtration chromatography and thin-layer chromatography of rodent and primate urine extracts within twenty-four hours postirradiation. Species variations between rodents and primates may account for different responses of fluorescent urinary products after irradiation. Author

Descriptors:

• (*RADIOBIOLOGY
• LABORATORY ANIMALS)
• RADIATION EFFECTS
• SUBLETHAL DOSAGE
• WHOLE BODY IRRADIATION
• URINE
• FLUORESCENCE
• CHROMATOGRAPHIC ANALYSIS
• PRIMATES
• RODENTS

Subject Categories:

• Radiobiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE