FLUOROMETRIC DETECTION OF BIOLOGIC CHANGES IN IRRADIATED LABORATORY ANIMALS.
Final rept. Oct 66-Nov 67,
NORTHROP CORPORATE LABS HAWTHORNE CALIF LIFE SCIENCES LABS
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The excretion of fluorescent products by rodents and primates after exposure to whole-body sublethal 60Co gamma irradiation was investigated. Gel filtration chromatography and thin-layer chromatography were used to fractionate and quantitate radiation-responsive fluorescent urinary products. A major advancement in quantitating fluorescent urine products was achieved by the fluorescence-scanning of thin-layer chromatograms. Certain radiation dose-response relationships were measurable in two major fluorescent fractions from gel filtration chromatography and thin-layer chromatography of rodent and primate urine extracts within twenty-four hours postirradiation. Species variations between rodents and primates may account for different responses of fluorescent urinary products after irradiation. Author