A STUDY OF THE DEVELOPMENT OF THE TICK-BORNE ENCEPHALITIS VIRUS IN HUMAN AND ANIMAL TISSUE CULTURES
ARMY BIOLOGICAL LABS FREDERICK MD
Pagination or Media Count:
The tick-borne encephalitis virus is successfully reproduced in tissue cultures of the human embryo, HeLa cells, monkey and dog kidney tissue, skin-muscle tissue of white mice and rats, and in cultures of the various tissues of the chick embryo. The maximum viral titers in all of the tissue cultures are observed on the 3rd-8th day after inoculation. The most intensive propagation of the virus is noted in the cultures of the human skin-muscle tissue and the HeLa cells. A cytopathogenic effect is registered regularly in the cultures of human skin-muscle and kidney tissued on the 2nd-4th day after inoculation. The viral titers determinable according to the cytopathogenic effect coincide with the titers on the mice. The viruss cytopathogenic does not occur in the presence of serum from a tick-borne encephalitis patient. A cytopathogenic effect is registered in some passages in cultures of the embryonic skin-muscle tissue of the white rat. The viruss cytopathogenic effect is not developed in cultures of human lung tissue, HeLa cells, monkey and dog kidney tissue, skin-muscle tissue of white mice, and tissues of the chick embryo.