AN INSULIN-INHIBITORY FACTOR OF HUMAN BLOOD.
Rept. for Nov-Dec 67,
SCHOOL OF AEROSPACE MEDICINE BROOKS AFB TEX
Pagination or Media Count:
An insulin inhibitor has been found in human blood through use of a two-antibody radioimmunoassay. This report presents additional data and compares the inhibitor to other reported inhibitors. Treatment with heat, strong base, or disodium ethylenediamine tetraacetate EDTA does not remove inhibition therefore, the factor is probably not complement C. At present, it cannot be determined whether the inhibitor acts between insulin and the first antibody, or between the first and the second antibody. Dilution of the sample 110 completely removes the inhibition. Recovery curves over a range of 50 to 500 micro units of insulin show 100 recovery at all points. Author