Accession Number:

AD0669787

Title:

CHEMICAL RADIOPROTECTION OF HEMOPOIETIC COLONY-FORMING CELLS: COMPARATIVE EFFECT OF AET, ANOXIA AND URETHAN,

Descriptive Note:

Corporate Author:

NAVAL RADIOLOGICAL DEFENSE LAB SAN FRANCISCO CALIF

Personal Author(s):

Report Date:

1968-04-04

Pagination or Media Count:

24.0

Abstract:

The hemopoietic colony-forming unit CFU technique of Till and McCulloch was employed to test the radioprotective effect of AET, anoxia and urethan on marrow cells irradiated in vivo. For AET and anoxia, a dose-reduction factor of 1.9 to 2.1 was found. Since the marrow cells were assayed for CFU content immediately after irradiation of the donor, the observed effect can be interpreted as a true radiation dose reduction. By contrast, urethan injection did not increase the survival of marrow CFU assayed immediately after whole-body X irradiation. However, urethan as well as AET, afforded radioprotection of endogenous CFU content of spleen and bone marrow, but not of endogeneous spleen colony count. It is concluded that the mechanism of radioprotection by urethan is fundamentally different from that of AET or anoxia. The data also suggest that mouse bone marrow CFU content, assayed immediately after irradiation, does not necessarily correlate with animal survival. Author

Subject Categories:

  • Radiobiology
  • Pharmacology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE