Accession Number:

AD0669786

Title:

ANTIBODY FORMATION BY TRANSFERRED PERITONEAL CELLS AND SPLEEN CELLS OF MICE. I. TRANSFER OF CELLS FROM IMMUNIZED NON-IRRADIATED DONORS TO SYNGENEIC RECIPIENTS WITH AND WITHOUT ANTIGEN,

Descriptive Note:

Corporate Author:

NAVAL RADIOLOGICAL DEFENSE LAB SAN FRANCISCO CALIF

Personal Author(s):

Report Date:

1968-03-22

Pagination or Media Count:

46.0

Abstract:

Peritoneal cells and spleen cells from LAF 1 mice given 3 intraperitoneal immunizations of sheep red blood cells synthesized hemagglutinins after transfer to x-irradiated syngeneic recipients, either with or without a concomitant injection of antigen. Antibody formation by cells transferred with antigen resembled a secondary antibody response in intact animals. Hemagglutinins appeared rapidly and in high titer. Approximately 50 of the antibodies were resistant to treatment with 2-mercaptoethanol. Antibody formation by cells transferred without further exposure to antigen differed in several respects. Hemagglutinin titers were lower. Throughout the period of observation, only 6-25 of the antibodies formed were mercaptoethanol-resistant. In recipients injected with spleen cells, antibodies appeared rapidly, suggesting that mature antibody-forming cells had been transferred. However, in recipients injected with peritoneal cells from the same donors, antibodies were detected only after a delay of several days, which suggested that mature antibody-forming cells had not been transferred. Hemagglutinins were synthesized as well by a suspension containing all types of peritoneal cells as by a fraction consisting almost entirely of lymphoid cells. Recipients of peritoneal cells from donors which had been given either 1 intraperitoneal immunization or 3 intravenous immunizations had no or only low hemagglutinin titers, in contrast to recipients of cells from donors given 3 intraperitoneal immunizations. Author

Subject Categories:

  • Radiobiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE