METABOLISM OF C 1 COMPOUNDS BY MICROORGANISMS, PART II.
Final rept. (Part 2),
SHEFFIELD UNIV (ENGLAND) DEPT OF MICROBIOLOGY
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An assay was developed for the enzyme system in methane-grown Pseudomonas methanica which catalyses the condensation of formaldehyde with ribose-5-phosphate to give a hexose phosphate. Using this assay system, the enzyme system was partially purified by gel-filtration and ion exchange chromatography. Studies were made of mutants of Pseudomonas AM1 deficient in their ability either to grow on C 1 compounds or to synthesize serine or glycine from succinate. The metabolism of Pseudomonas oxalaticus in mixtures of formate and certain C 2 growth substrates was determined. Author