HISTOLOGIC REACTION OF ADULT RAT SKIN AFTER 13 MEV PROTON IRRADIATION AND ITS VIABILITY IN CELL CULTURE
Rept. for May 1964-Jun 1966
SCHOOL OF AEROSPACE MEDICINE BROOKS AFB TX
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The carcinogenic effects of low energy 13 Mev protons on adult rat skin were studied in two phases Phase 1--A pathologic study in which the oncogenesis of proton irradiation was determined eight months post irradiation. Phase 2--Cell culture experiments based on the hypothesis that growth and viability of adult skin cells irradiated in vivo and later cultured in vitro would reveal carcinoma potential before actual tumor formation could be detected. In phase 1, one animal followed the anticipated pattern nine months post irradiation. It was biopsied for culture six times and in each instance gave a consistent growth in vitro. This animal later developed a basal cell carcinoma. In phase 2, no animals provided viable cells in culture at intervals of 5, 30, or 60 days post irradiation. The interval between 60 days and eight months post irradiation unfortunately could not be followed in this study.