AUTOMATED DETERMINATION OF HEMOGLOBIN IN PLASMA.
Rept. for Oct 66-Sep 67,
SCHOOL OF AEROSPACE MEDICINE BROOKS AFB TEX
Pagination or Media Count:
A method is described for the automated determination of plasma hemoglobin levels in the range of 0.1 mg. to 1.5 mg. using the well-known benzidine-peroxidase reaction. The coefficient of variation, based on day-to-day reproducibility of procedure, is less than 10. In recovery experiments, values of 93 to 98 were obtained. A comparison of the automated procedure with a reference manual technic reveals that the mean of assay values on dog subjects obtained with the automated procedure is 0.042 mg. higher than the mean obtained with the reference method. None of the assay values, however, are excluded by the plus or minus com3 combined S.D. limits for the two procedures. The automated procedure appears to be comparable to the manual procedure in terms of accuracy and precision and superior with reference to convenience, rapidity of assay, and reliability. Author