STUDIES OF THE MAMMALIAN BRAIN FUNCTION IN VITRO.
Final rept. no. 2, Sep 66-Sep 67,
KOBE UNIV (JAPAN) SCHOOL OF MEDICINE
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Viability of the frozen cat brain was studied by electrocorticograms, spontaneous single nerve cell discharges, light and electronmicroscopic examination. The brain which had been perfused with 4 percent dextran Hanks solution in situ was isolated and cryoprotective agents were added in the perfusate. Thereafter it was frozen slowly and stored at -20C for more than 2.5 years. The results of electrical and microscopic examinations were coincided with each other. Tissue defects were observed sporadically in electronmicroscopic photographs of the brain which had been stored for 50 days, but it was difficult to find distinct abnormality in electrical recordings. Electrocorticograms were recorded even from the brain frozen for 203 and 777 days, but the wave was slower in the former and remarkably monotonous oscillation was observed in the latter and no record was obtained from the brain frozen for 952 days. These results were also supported by morphological examination which showed some unidirectional deterioration had been proceeded during the frozen storage. Cryoprotective effect of glycerol, dimethylsulfoxide, hydrogenated dextran and polyvinylpyrrolidone was also compared. Author
- Anatomy and Physiology