CHROMOSOMAL CHANGES ASSOCIATED WITH ALTERED BIOLOGICAL CHARACTERS IN HOMOLOGUS TRANSPLANTS.
Final rept. no. 4, Dec 66-Dec 67,
SASAKI FOUNDATION MEDICAL INSTITUTION TOKYO (JAPAN)
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The Yoshida sarcoma, an ascites tumor of the rat, whose character had shown itself stable during more than 15 years of homologous transplantation, underwent marked changes when inoculated to immunologically conditioned host animals, giving rise to different tumor cell populations with altered biological characteristics. Variant tumor cells thus developed, demonstrated the property to form islands or bonding of tumor cells in ascitic fluid, while the original Yoshida sarcoma cells had never shown any sign of cell-cluster formation. The host animals transplanted with the variants survived longer than those with the original Yoshida sarcoma cells, i.e. the variants showed decreased malignancy. These characteristics of the variants were maintained stable during further serial passage of them in normal homologous hosts. In this study, eight variant cell lines of the Yoshida sarcoma were submitted to examination of their chromosome constitution and Feulgen-microspectrophotometric determination of DNA amount of their metaphase nuclei. These variants differed from each other and from the original Yoshida sarcoma in their chromosome features, except for three variants with the same chromosome number and DNA amount as well as a general likeness of their idiograms. A rough parallelism between the modal chromosome number and DNA content of these variants and their original Yoshida sarcoma was seen. Author
- Medicine and Medical Research