ANTIGENICITY OF CONJUGATED PEPTIDES AND RELATED TOXIC MOLECULES. ELEDOISIN AND RELATED PEPTIDES AS HAPTENS.
Semiannual status rept. 1 Jul-15 Nov 67,
MIDWEST RESEARCH INST KANSAS CITY MO
Pagination or Media Count:
Studies were initiated to test the feasibility of producing effective neutralizing antisera to low molecular weight toxins by using these antigens as haptens. Two approaches are planned 1 Adsorption of the antigen to an especially prepared polymethylmethacrylate resin and subsequent injection into the rabbit. 2 Conjugation of the antigen to a larger molecule, poly-L-lysine or poly-L-alanine, as examples, prior to injection. The report describes the experimental approach to the problem. Immunizations were initiated with approach number 1. Precipitating antibodies as detected by gel diffusion have not yet been produced to the peptide bradykinin. As explained in this report, bradykinin is being used as a prototype molecule for the toxins, eledoisin and saxitoxin, because of the scarcity of these two toxins. Author