UNIQUE METHODS OF VIRAL COLLECTION AND IDENTIFICATION
Final rept. 1 Jan 1966-30 Jun 1967
MIDWEST RESEARCH INST KANSAS CITY MO
Pagination or Media Count:
Six unique methods for the rapid identification of virus have been devised and investigated to determine their potential suitability for use in the fabrication of a new virus identification system. In the phagocytosis in vitro studies we prepared immune WBC and observed their response when challenged with virus. Impedance measurements on phospholipid bilayer membranes in buffer solutions have been used to detect the immune reaction. The effects of virus challenge on the electrophoretic migration rate of antibody coated plastic particles and tanned sheep red blood cells have been measured. Hydrogen overvoltage measurements have been performed on solutions containing antibody and antigen-antibody complexes. Fluorescent tagged antibody has been employed in the single and double immunodiffusion techniques in an effort to visualize the soluble virus-antibody conjugates. Virus has been tagged with rhodamine to permit its use with immobilized antibody for a viral identification process. Molecular sieves and ion exchange resins have been employed in studies for the separation and detection of soluble virus-antibody complexes.