THE EFFECT OF SOME PROTECTIVE SUBSTANCES ON THE PROCESS OF FREEZING AND THAWING OF CELLULAR ELEMENTS IN THE BLOOD.
Figs. refs. omitted,
NAVAL MEDICAL SCHOOL BETHESDA MD TRANSLATION SERVICE
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Damage to blood cells in blood taken with the usual glucose-citrate solution with out the addition of protective substances, occurs during the process of freezing and thawing, apparently, occurs essentially during the stage of thawing. Ethyl alcohol, used as a protective substance, in a complex glucose-citrate solution, protects to a certain degree, the blood cells during the process of freezing and thawing in spite of intensive re-crystallization, even at relatively low temperature. In the concentrated solution, containing two protective substances ethyl alcohol and glycerine, the process of recrystallization is weak. At temperatures to -50C a considerable portion of the solution remains non-crystallized and fills up the canals between the crystals in which there are present some mobile cells. Author
- Anatomy and Physiology