Accession Number:

AD0658850

Title:

THE EFFECT OF SOME PROTECTIVE SUBSTANCES ON THE PROCESS OF FREEZING AND THAWING OF CELLULAR ELEMENTS IN THE BLOOD.

Descriptive Note:

Figs. refs. omitted,

Corporate Author:

NAVAL MEDICAL SCHOOL BETHESDA MD TRANSLATION SERVICE

Personal Author(s):

Report Date:

1967-08-11

Pagination or Media Count:

16.0

Abstract:

Damage to blood cells in blood taken with the usual glucose-citrate solution with out the addition of protective substances, occurs during the process of freezing and thawing, apparently, occurs essentially during the stage of thawing. Ethyl alcohol, used as a protective substance, in a complex glucose-citrate solution, protects to a certain degree, the blood cells during the process of freezing and thawing in spite of intensive re-crystallization, even at relatively low temperature. In the concentrated solution, containing two protective substances ethyl alcohol and glycerine, the process of recrystallization is weak. At temperatures to -50C a considerable portion of the solution remains non-crystallized and fills up the canals between the crystals in which there are present some mobile cells. Author

Subject Categories:

  • Anatomy and Physiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE