EXAMINATION FOR COMPLEMENT-REQUIRING NEUTRALIZING ANTIBODIES AGAINST JAPANESE ENCEPHALITIS, WESTERN EQUINE ENCEPHALITIS AND VACCINIA VIRUSES
Final rept. 25 Jun 1966-24 Jun 1967
YOKOHAMA MUNICIPAL UNIV (JAPAN) FACULTY OF MEDICINE
Pagination or Media Count:
It was planned to examine whether the early serum of animals immunized with Western equine encephalitis WEE, Japanese encephalitis JE and vaccinia viruses contained complement-requiring neutralizing CRN antibody. A prerequisite to this experiment was to establish a simple neutralization test procedure which could titrate antibody titers of many serum samples at one time. Umeda et als agar cover slip technique used for herpes virus neutralization was applied to WEE and JE viruses. When reaction mixtures were placed at 4 deg C overnight and then at 37 deg C for 3 hours before inoculation to cells, a clear endpoint could be obtained. With vaccinia virus, this method is not yet applicable. By the use of the above method, sera of guinea pigs immunized with WEE virus were subjected to neutralization tests in the presence and absence of 10 units complement, and it was shown that the CRN antibody appeared in the 2nd week serum. An exact estimation of the first detectable time of this antibody is being studied now. It seems that the appearance of the CRN antibody precedes that of HI and CF antibodies. In the cases of JE and vaccinia viruses, preliminary tests using rabbits clearly showed that the CRN antibodies appeared in early serums, and detailed tests are being done at present to determine the exact time of its appearance and the immunochemical nature of the CRN antibodies thereby obtained.