USE OF GERMFREE ANIMALS IN THE STUDY OF DENTAL CARIES.
Final technical rept. 1 Aug 62-31 Jul 65,
CHICAGO UNIV ILL ZOLLER MEMORIAL DENTAL CLINIC
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Over the years, it has been possible to demonstrate that in the complete absence of all bacteria, germfree rats could not develop dental decay even though ingesting a caries-inducing diet for as long as 150 days when the early experiments were terminated. Later, in the presence of an enterococcus Streptococcus sp isolated from a rat in the external environment and with dental caries, otherwise germfree rats developed caries, though a longer time period was involved. Both in one early group of rats, as well as in several later groups of otherwise germfree rats inoculated with a lactobacillus strain, originally isolated from a human mouth with caries, dental caries did occur in some rats molar teeth, but especially so in subsequent generations of rats living in such a lactobacillus environment. Inoculated leptotrichia-like filamentous bacteria were difficult to establish and failed to induce caries in otherwise germfree gnotobiotic rats. Finally, in recently-observed animals maintained their life span on caries-inducing diets, no dental decay could be detected, thus conclusively demonstrating the etiological relationship of bacteria and dental caries in this kind of unique study. Author
- Animal Husbandry and Veterinary Medicine
- Anatomy and Physiology
- Medicine and Medical Research