THE ROLE OF PUTRESCINE IN MICROBIAL METABOLISM.
Final rept. 1 Sep 65-31 Aug 66,
NEW HAMPSHIRE UNIV DURHAM
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A sensitive and reliable quantitative method for the analysis of naturally-occurring polyamines was developed. The method is applicable to animal and plant tissues, insects and bacteria. The procedure will detect 0.5 millimicromole of putrescine, spermidine and spermine and is a fast and accurate method for the analysis of the polyamines by thin-layer chromatography. Polyamines react with nucleic acids and the contract research has extended knowledge on the mechanism and possible biological function of polyamine-nucleic acid interactions. Polyamines inhibit the degradation turnover of ribosomes at low concentrations, at high concentrations of polyamines, the turnover of ribosomes is stimulated. Both inhibitory and stimulatory concentrations of polyamines are within the limits of the occurrence of these polycations in living cells. The polyamines act synergistically with Mg ion in the stabilization of the functional ribosome particles which are essential for protein synthesis. The polyamines compete with K ion, which increases the proportion of inactive ribosome particles. The research has contributed to the important knowledge of regulatory mechanisms in growth processes. Author
- Anatomy and Physiology