DIFFERENTIATION, CLASSIFICATION AND LABORATORY DIAGNOSIS OF EL TOR VIBRIOS.
Final rept. 15 Mar 66-14 Mar 67,
KYUSHU UNIV FUKUOKA (JAPAN) DEPT OF BACTERIOLOGY
Pagination or Media Count:
By the use of kappa-type phage, El Tor cholera vibrios were classified into Celebes type and Classic-Ubon type, and the Celebes type was further divided into Original and Cured strains. This phage-typing was applied to epidemiological studies. Biologic properties of Cured strains and properties of phages liberated from Inaba type El Tor cholera vibrios and some Asiatic cholera vibrios were clarified. The kappa-type phage could be liberated from spheroplast form of Celebes type El Tor cholera vibrios cells, and its significance was discussed in relation to the detection of long-term carriers. Successful results of the application of phage detection method to carrier studies were described, and furthermore, a new phage-enrichment method for detection of long-term carriers was devised. The fluorescent antibody stainability of flagellum of El Tor cholera vibrio after heating or trichloracetic acid treatment was examined. Author