NEUROPHYSIOLOGICAL STUDIES ON THE OLFACTORY RECEPTIVE MECHANISM.
Final rept. no. 1, 21 Jan 66-20 Jan 67,
GUNMA UNIV MAEBASHI (JAPAN)
Pagination or Media Count:
Ionic mechanism of the negative receptor potentials EOG was studied in the olfactory epithelium of the frog by substituting Na in bathing Ringers solution with other ions or chemicals, or by increasing or decreasing K or Ca in the solution. It was found that any of sucrose, Li, choline, tetraethyl ammonium TEA and hydrazine can not replace Na in maintaining the EOGs. When Na and K in Ringers solution were reversed, the negative EOGs reversed their polarity. Then, the reversed EOGs showed a complete or nearly complete recovery only in normal Na Ringers solution but never in the other solutions. Thus, essential role of Na was proved. In K-free Ringers solution, the negative EOGs decreased in amplitude after an initial increase. In K-excess Ringers solution, the EOGs increased in amplitude. Thus, contribution of K to the negative EOGs was proved. Contribution of Cl- to the EOGs was ruled out by the selectively depresent action of Ba on the Cl- permeability. It is concluded that the negative EOGs are elicited by increase in permeability of the olfactory receptive membrane to Na and K, but not to Cl-. Author
- Anatomy and Physiology