MEASUREMENT OF SERUM IONIC CALCIUM USING A SPECIFIC ION ELECTRODE.
Rept. for Aug 66-Jan 67,
SCHOOL OF AEROSPACE MEDICINE BROOKS AFB TEX
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A recently developed calcium ion electrode was evaluated for use in the routine measurement of serum ionic calcium. The electrode, used in conjunction with an expanded scale pH meter, permits the direct and accurate assay of ionic calcium in fresh serum samples with a minimal expenditure of time and materials. Factors found to be critical to reliable operation include electrode mounting configuration, diluent for the calibration standards, sample preparation, control of pH, uniformity of sample volume and thermostasis. Analytic precision--a function of electrode placement, instrumental stability and readout parallax--is routinely excellent. Discrimination and recovery studies indicate the minimal assayable concentration differences to be between 0.2 and 0.3 mg.. The electrode appears to be applicable to a wide variety of clinical and nonclinical investigations. Author
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