Accession Number:

AD0653748

Title:

FATIGUE AND STRESS STUDIES: AN IMPROVED SEMI-AUTOMATED PROCEDURE FOR FLUOROMETRIC DETERMINATION OF PLASMA CATECHOLAMINES,

Descriptive Note:

Corporate Author:

CIVIL AEROMEDICAL INST OKLAHOMA CITY OKLA

Personal Author(s):

Report Date:

1966-04-01

Pagination or Media Count:

13.0

Abstract:

A semiautomated technique is described for the estimation of total catecholamines in plasma by the trihydroxyindole procedure. The method utilizes conventional alumina-column chromatography for isolation of the amines. Catecholamine oxidation, tautomerization, and fluorescence detection are accomplished by an automated chemical system. Recoveries from plasma of 0.01 and 0.02 micrograms total catecholamines10-ml sample were 86.4 plus or minus 8.9 SD and 85.6 plus or minus 5.8 SD, respectively. Total catecholamine level in resting human plasma, determined by the present technique, was 0.89 plus or minus 0.15 SD microgramsl. Author

Subject Categories:

  • Biochemistry
  • Stress Physiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE