EARLY DETECTION OF ARBOVIRUSES IN TISSUE CULTURES BY HEMAGGLUTINATION
ARMY BIOLOGICAL LABS FREDERICK MD
Pagination or Media Count:
The hemagglutination reaction with the virus of Venezuelan encephalomyelitis and the Western variant of American equine encephalomyelitis, incubated in tissue cultures of chick fibroblasts, takes place at 4C in a pH zone of 5.4--6.6 optimum pH 5.8--6.0. During infection of the cultures with large doses of virus within the limits of 10 to the 7th power to 10 to the 9th power CPD50 the hemagglutinins appear in the cultural fluid in 5--6 hours following infection, and the cytopathic effect is detected after 24 hours. The hemagglutination phenomenon may be used for the early detection of a virus in cultures.