FLUORESCENCE OF ACRIDINE ORANGE COMPLEXES WITH BASES AND NUCLEOTIDES
ARMY BIOLOGICAL LABS FREDERICK MD
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The parallelism between the changes in the values of fluorescence and duration, taking place with the formation of complexes of AO with DNA, testifies to the fact that during combination there is a lessening of the probability of heat dissipation of energy from the electronic excitation of the AO molecule during the time of its stay in the excited condition. Apparently this is the result of some specific interaction between the electronic systems of the dye and of the bases of DNA. It is possible that such an interaction plays a role in the specific bacteriostatic and mutagenic action of AO. It is of interest to determine whether this interaction takes place with all four nitrogen bases of DNA or just with some of them. An investigation was made of the fluorescence of solutions of AO, to which were added adenine, dimethyladenine, adenosine, adenosine triphosphate, thymine, uracil and cytosine, and also a purine compound -- caffeine.