MULTIPLE INFECTION OF CELL MONOLAYERS BY VIRUS MIXTURES
FORT DETRICK FREDERICK MD
Pagination or Media Count:
In studies of viral interference or mixed infections, difficulty is frequently encountered in distinguishing the specificity of cellular infections that result from the introduction of more than one virus onto cell monolayers. This may limit the type and number of agents that may be employed, particularly when the viruses evoke similar or indistinctive cellular responses. Quantitation of virus infectivity in the presence of another agent often involves intricate assay techniques. To facilitate experimental investigations at the cellular level, this report describes a rapid and highly specific procedure for distinguishing and quantitating virus infectivity by selective immunofluorescent staining in conjunction with fluorescent-cell counting.