Accession Number:

AD0651379

Title:

A STUDY OF BOTULIN TOXINS AND TOXOIDS BY FILTRATION THROUGH GEL. REPORT 1. EXAMINATION OF BOTULIN TOXINS AND TOXOIDS OF THE A AND C TYPES BY FILTRATION THROUGH SEPHADEX G-100

Descriptive Note:

Corporate Author:

FORT DETRICK FREDERICK MD

Personal Author(s):

Report Date:

1966-10-01

Pagination or Media Count:

11.0

Abstract:

By filtration through Sephadex G-100 dextran gel it is possible to separate type A and C botulin toxins and toxoids into fractions. The first fraction isolated by filtration through gel contained proteins with a molecular weight greater than 100,000 it corresponded to the antitoxin-binding activity of the toxoids and the partial lethal activity of toxins. Filtration through gel makes it possible to separate botulin toxins and toxoids into fractions which are different in their antigenic spectrum. By filtration of botulin toxoids through Sephadex gel it is possible to purify the active fractions from ballast low molecular compounds.

Subject Categories:

  • Biochemistry
  • Toxicology
  • Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE