THE VITALITY OF FLUORESCENCE MICROSCOPIC INVESTIGATIONS CARRIED OUT WITH THE HELP OF THE FLUOROCHROMES OF ACRIDINE ORANGE AND 3,4 BENZPYRENE,
ARMY BIOLOGICAL DEFENSE RESEARCH CENTER FREDERICK MD
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Cytological and autoradiographic methods were used to investigate the degree of vitality of certain types of fluorescence microscopic investigation of living cells. On the basis of tests, carried out with acridine orange fluorochrome, a classification is proposed for the types of fluorescence microscopic investigation Vital fluorochrome hardly reflected in the metabolism and viability of the cell supravital fluorochromed cell preserves the majority of features of a living cell, but not all of them, in particular such a treatment of cells with acridine orange leads to inhibition of protein synthesis pseudovital the cell dies as a result of the action of fluorochrome. It was established that when working with a culture of cells of amniotic epithelium strain FL, acridine orange in a concentration of 0.3--0.5 microgramsml makes it possible to conduct a vital study, and in a concentration of 10--20 microgramsml a supravital study of the cells. With the help of 3,4- benzpyrene in a concentration of 15--25 microgramsml it is possible to make a vital investigation of the distribution of lipids in the same cells. The stated concentrations refer to cells which have been shielded from the action of light.