COMPARATIVE CYTOFLUOROMETRIC STUDY OF THE DNA CONTENT IN HIGH POLYPLOIDY CELL NUCLEI STAINED WITH ACRIDINE ORANGE AND FLUORESCENT SCHIFF-TYPE REAGENT
ARMY BIOLOGICAL DEFENSE RESEARCH CENTER FREDERICK MD
Pagination or Media Count:
An investigation of the macronucleus of Tetrahymena pyriformis, stained with fluorescent Schiff-type reagent auramine 0-SO2 in the Feulgen reaction, showed that the cytofluorometric method may be used to determine the relative amounts of DNA even in high polyploidy cellular nuclei. When RNA has been removed from the cells it is possible to judge the condition of the DNA-protein based on measurements of the intensity of fluorescence of the cell nuclei, stained with acridine orange. The two methods are complementary in the investigation of cell nuclei by the cytofluorometric method.