BIOSYNTHESIS OF STAPHYLOCOCCAL PHAGE ON CELL FREE BACTERIAL MEDIA
ARMY BIOLOGICAL LABS FREDERICK MD
Pagination or Media Count:
Up until now it was considered solidly established that viruses and phages as obligate parasites may multiply only in living cells which are susceptible to the given virus or phage. However, in 1961 the possibility of the synthesis of the poliomyelitis virus from viral RNA in the system of destroyed cells of a monkey kidney with the addition of certain amino acids, nitrogenous bases and enzymes was reported. In 1962 reports were published on the multiplication of the tobacco mosaic virus in a system which did not contain living cells. Various nucleotides adenosine-, guanosine-, cytidine- and uridene-triphosphates were entered into this system. The addition to such a medium of the purified sap from plants which were afflicted with the tobacco mosaic virus causes the rapid multiplication of new viral particles. In this way the biosynthesis of viruses on media which do not contain living cells was demonstrated. The tests reported in this paper, on the biosynthesis of staphylococcal phage, were conducted on cell-free bacterial lysates.