Accession Number:

AD0650363

Title:

FETAL LIVER CELLS: A SOURCE OF SPECIFIC IMMUNOGLOBULIN PRODUCTION IN RADIATION CHIMERAS,

Descriptive Note:

Corporate Author:

NAVAL RADIOLOGICAL DEFENSE LAB SAN FRANCISCO CALIF

Personal Author(s):

Report Date:

1967-02-24

Pagination or Media Count:

17.0

Abstract:

Adult-thymectomized and intact C57L x AF1 mice were X-irradiated 870 rad, and injected ip with 20-33 x 10 to the 6th power nucleated cells from livers of 14-21 day C57B16 x C57B16 embryos. In other experiments the mice received 300,000 syngeneic bone marrow cells iv, and dissociated gut cells from 14-21 day C57B16 x C57B16 embryos ip. The sera from these chimeras were assayed for the presence of donor-type gamma-globulins. These mice were also sensitized to a synthetic polypeptide TGAL, and their sera tested for total and for donor-type anti-TGAL antibody. All the mice, thymectomized as well as intact, which received fetal liver cells, had significant quantities of donor-type gamma-globulins in their sera by 58 days postirradiation. Only 1 of 14 mice injected with embryonic gut cells was found to have donor-type gamma-globulins by 95 days postirradiation. Following immunization with TGAL, the fetal liver cell chimeras intact thymus exhibited specific antibody 1010, while none of the thymectomized chimeras 021 were positive for this antibody. In the immunized, intact-thymus group, significant amounts of the specific anti-TGAL antibody was of donor origin. Thus, mouse fetal liver, as early as the 14th day of gestation, contains lymphoid stem cells capable of immunoglobulin synthesis in the absence of thymus. However, their ability to form certain specific antibodies is dependent upon intact thymic function. The results suggest that the donor-type immunoglobulins synthesized in the absence of the thymus may be nonsense of defective antibody-like molecules. Author

Subject Categories:

  • Medicine and Medical Research
  • Radiobiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE