THE EFFECT OF OXYGEN ON DOG PLASMA SULFHYDRYL GROUPS IN VITRO.
Rept. for Jul 65-Mar 66,
SCHOOL OF AEROSPACE MEDICINE BROOKS AFB TEX
Pagination or Media Count:
A method is described in which biologic fluids are continuously exposed to a test atmosphere, and sampling is achieved without disruption of the atmosphere. Samples are withdrawn at intervals and analyzed for sulfhydryl content by amperometric titration with the rotating platinum electrode using AgNO3 as the titrant. Inasmuch as this project was undertaken to study the effect of oxygen on sulfhydryl-containing enzymes in vitro, the system was first evaluated using dog plasma as the source of protein. The decrease in dog plasma sulfhydryl content over a 4-hour exposure was 9.3 for nitrogen, 24.7 for air, and 39.2 for oxygen. Author
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