THE INFLUENCE OF IRON ON THE VIRULENCE OF THE PLAGUE MICROBE FOLLOWING PASSAGING OF THE CULTURE ON ARTIFICIAL NUTRIENT MEDIA
ARMY BIOLOGICAL LABS FREDERICK MD
Pagination or Media Count:
Following 30 successive passages on artificial nutrient media, containing increased concentrations of ferrous salt, the virulence of plague microbe culture 138, following passaging under aerobic conditions, did not change noticeably. Following passaging under anaerobic conditions, in some cases a lowering of virulence was noted. Incubation under anaerobic conditions on media containing guinea pig blood as the nutrient substrate promoted a certain increase in virulence.