QUANTITATIVE ASSESSMENT OF PHAGOCYTOSIS BY MACROPHAGES IN VITRO OF ANTHRAX BACILLI OF DIFFERENT VIRULENCE. REPT. 1. PRINCIPLES OF THE METHOD AND ITS RELIABILITY
ARMY BIOLOGICAL LABS FREDERICK MD
Pagination or Media Count:
For the purpose of determining the reliability of the data which was obtained with the help of the proposed method for the quantitative evaluation of the reaction of phagocytosis, an analysis was carried out on accuracy, taking into consideration the following factors which are capable of influencing the results of the calculations 1 accuracy of the observance of the initial doses of the cellular and bacterial population, 2 degeneration of macrophages during cultivation, 3 unavoidable deviations in the time for taking the preparations from the test tubes, 4 errors committed when sketching the enlarged sector of the preparation and when conducting measurements. The method developed makes it possible to quantitatively assess the phagocytosis of anthrax bacilli with a various degree of virulence in a culture of macrophages. The results obtained were reliable.