A COMPARATIVE STUDY OF THE HEMOLYTIC PROPERTIES OF SOME MICROORGANISMS
ARMY BIOLOGICAL DEFENSE RESEARCH CENTER FREDERICK MD
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The lipids of Bact. pestis, Bact. pseudotuberculosis, B. anthracis STI and E. coli, as well as acetone-soluble fractions of these lipids are capable of causing lysis of washed-off erythrocytes of the guinea pig. The lipids of Staph. aureus are devoid of hemolytic properties. The starting lyophilized cultures of the aforesaid microbes, except Bact. pestis and Bact. pseudotuberculosis are hemolytically inactive. The hemolytic properties of lyophilized pseudotuberculous bacteria manifest themselves after a more or less prolonged time after lyophilization. The water-insoluble residues of these bacteria from the very beginning display comparatively well-pronounced hemolytic properties, just as the water-insoluble residue of Bact. pestis. The hemolytic activity of all bacterial lipids tested is similar to the hemolytic activity of oleic and stearic acids. This confirms the assumption that plague hemolysin and hemolysins of other microbes tested, concentrated in lipids of the latter, are apparently higher fatty acids, mainly of the saturated series.